【学歴】
山梨大学大学院医学工学総合教育部博士課程先進医療科学専攻修了　博士（医学）

【職歴】
山梨医科大学（現：山梨大学）附属病院　検査部
群馬パース大学　保健科学部　検査技術学科（現：医療技術学部　検査技術学科）

【資格】
臨床検査技師，1級遺伝子分析科学認定士

遺伝子検査学，分子生物学，血液学（血小板）

血小板活性化と血管病態のクロストーク

【researchmap】
https://researchmap.jp/osada-yamanashi-paz

• Phosphopeptide enrichment using Phos-tag technology reveals functional phosphorylation of the nucleocapsid protein of SARS-CoV-2. Yoko Ino, et al. Journal of Proteomics, Volume 255, 104501, 2022.
• Evaluation of four phosphopeptide enrichment strategies for mass spectrometry-based proteomic analysis. Yoko Ino, et al. PROTEOMICS, Vol 22(7), e2100216, 2022.
• Phos-tag diagonal electrophoresis precisely detects the mobility change of phosphoproteins in Phos-tag SDS-PAGE. Okawara Y, et al. J Proteomics. 16;231:104005, 2021.
• Soluble CLEC-2 is generated independently of ADAM10 and is increased in plasma in acute coronary syndrome: comparison with soluble GPVI. Inoue O, et al. Int J Hematol. Vol.110(3):285-294, 2019.
• Cobalt-hematoporphyrin inhibits CLEC-2–podoplanin interaction, tumor metastasis, and arterial/venous thrombosis in mice. N Tsukiji, et al. Blood adv. Vol11;2(17):2214-2225, 2019.
• Platelet CLEC-2: Roles Beyond Hemostasis. Suzuki-Inoue K, et al. Semin Thromb Hemost.Vol.44(2):126-134, 2019.
• Functional characterization of recombinant snake venom rhodocytin: rhodocytin mutant blocks CLEC-2/podoplanin-dependent platelet aggregation and lung metastasis. Sasaki T, et al. J Thromb Hemost. Vol.16(5). 960-972. 2019.
• C-type lectin-like receptor 2 promotes hematogenous tumor metastasis and prothrombotic state in tumor-bearing mice. Shirai T, et al. J Thromb Hemost. Vol.15 (3) pp.513-525, 2017.
• Physiological and pathophysiological roles of interaction between CLEC-2 and podoplanin: partners from utero to adulthood. Suzuki-Inoue K, et al. J Thromb Hemost. Vol.15 (2) pp.219-229, 2017.
• Podoplanin-positive periarteriolar stromal cells promote megakaryocyte growth and proplatelet formation in mice by CLEC-2. Tamura S, et al. Blood. Vol.127 (13) pp.1701-1710, 2016.
• Vascular Smooth Muscle Cells Stimulate Platelets and Facilitate Thrombus Formation through Platelet CLEC-2: Implications in Atherothrombosis. Inoue O, et.al. PLoS One. 2015 sep 29;10(9):e0139357, 2015.
• Measurement of soluble C-type lectin-like receptor 2 in human plasma. Kazama F, et al. Platelets. Vol.26 (8) pp.711-719, 2016.
• Platelet-derived sphingosine 1-phosphate induces migration of Jurkat T cells. Iino J, et al. Lipids Health Dis. Vol.13 (1) pp.150-159, 2015.
• Platelet Activation Receptor CLEC-2 Regulates Blood/Lymphatic Vessel Separation by Inhibiting Proliferation, Migration, and Tube Formation of Lymphatic Endothelial Cells. Osada M, et al. J Biol Chem. Vol.287 (26) pp. 22241-22252, 2014.
• Causes of Thrombocytopenia in Chronic Hepatitis C Viral Infection. Osada M, et al. Clin Appl Thromb Hemost. Vol.18 (3) pp.272-280, 2014.
• Regulation by sphingolipids of the fate of FRTL-5 cells. Satoh Y, et al. J Biochem. Vol.145 (1) pp.31-36, 2009.
• Involvement of sphingosine 1-phosphate, a platelet-derived bioactive lipid, in contraction of mesangium cells. Osada M, et al. J Biochem. Vol.142 (3) pp.351-355, 2007.
• Fluid shear stress enhances the sphingosine 1-phosphate responses in cell-cell interactions between platelets and endothelial cells. Aoki S, et al. Biochem Biophys Res Commun. Vol.358 (4) pp.1054-1057, 2007.
• Sphingosine 1-Phosphate Receptor Expression Profile in Human Gastric Cancer Cells: Differential Regulation on the Migration and Proliferation. Yamashita H, et al. J Surg Res. Vol.130 (1) pp.80-87, 2006.
• Sphingosine 1-phosphate-related metabolism in the blood vessel. Aoki S, et al. J Biochem. Vol.138 (1) pp.47-55, 2005.
• The intracellular action of sphingosine 1-phosphate in GPVI-mediated Ca2+ mobilization in platelets. Ohmori T, et al. Thromb Res. Vol.115 (5) pp.409-415, 2005.
• Sphingosine 1-phosphate inhibits migration of RBL-2H3 cells via S1P2: cross-talk between platelets and mast cells. Yokoo E, et al. J Biochem. Vol.135 (6) pp.673-681, 2004.
• Independence of Tumor Necrosis Factor-α-induced adhesion molecule expression from sphingosine 1-phosphate signaling in Vascular Endothelial cells. Miura Y, et al. J Thromb Hemost. Vol 2 (6) pp.1019-1021, 2004.
• Platelet-derived sphingosine 1-phosphate induces contraction of coronary artery smooth muscle cells via S1P2. Ohmori T, et al. J Thromb Hemost. Vol. 2 (1) pp.203-205, 2004.
• Sphingosine 1-phosphate induces contraction of coronary artery smooth muscle cells via S1P(2). Ohmori T, et al. Cardiovasc Res. Vol. 58 (1) pp.170-177, 2003.
• Expression of the LIM Proteins Paxillin and Hic-5 in Human Tissues. Yuminamochi T, J Histochem Cytochem. Vol. 51 (4) pp.513-522, 2004.
• Modulation of sphingosine 1-phosphate/ EDG signaling by tumor necrosis factor-α in vascular endothelial cells. Osada M, et al. Thromb Res. Vol. 108 (2-3) pp.169-174, 2004.
• Enhancement of sphingosine 1-phosphate-induced migration of vascular endothelial cells and smooth muscle cells by an EDG-5 antagonist. Osada M, Biochem Biophys Res Commun. Vol.299 (3) pp.483-487, 2003.
• Wheat germ agglutinin-induced platelet activation via platelet endothelial cell adhesion molecule-1: involvement of rapid phospholipase C gamma 2 activation by Src family kinases. Ohmori T, et al. Biochemistry. Vol. 40 (43) pp.12992-13001, 2002.
• Involvement of Hic-5 in platelet activation: integrin aIIbb3-dependent tyrosine phosphorylation and association with proline-rich tyrosine kinase 2. Osada M, et al. Biochem J. Vol. 355 (Pt 3) pp.691-697, 2002.